| KINGJAMES | 400-year-old book (4,5,5) |
| JACOBEAN | 400-year-old material, account old boy's hidden away (8) |
| GREENLAND | 'This slow-moving one can live to be over 400 years old |
| PARIS | Home of the Pont Neuf ("New Bridge"), which is over 400 years old |
| MDCIII | 400 years ago |
| JAMESTOWN | American colony founded 400 years ago |
| JOHNPAULI | Shortest-reigning pope of the last 400 years |
| HAN | Chinese dynasty which ruled for over 400 years (3) |
| MDLII | 400 years before Ike's first term |
| NIGH | At hand, 400 years ago |
| DECADE | Kiki maybe grasping answer in 400 years (6) |
| TOMSK | City in Siberia dating from more than 400 years ago (5) |
| SICILIAN | The ___ Defence, chess strategy which has existed for more than 400 years (8) |
| YORK | See 43, This served as a theatre in England 400 years ago (3,4) |
| INN | This served as a theatre in England 400 years ago (3,4) |
| BOLOGNA | Italian city whose main square is dominated by the facade of Basilica di San Petronio, unfinished for over 400 years |
| CANARY | A popular pet bird who owes its coloration and vocal powers to 400 years of selective breeding by humans |
| INCA | Civilisation which flourished in South America for 400 years before the Spanish conquest (4) |
| KNOLE | Stately home in Kent, inhabited by the Sackvilles for some 400 years and often referred to as a calendar house for its legendary approximation of 365 rooms, 52 staircases, 12 entrances and seven court |
| WILLIAM | Although he died over 400 years ago, who is still quoted in everyday English? (7,11) |
